Ptprc (CD45RA) Mouse Monoclonal Antibody [Clone ID: OX-33]
CAT#: SM279P
Ptprc (CD45RA) mouse monoclonal antibody, clone OX-33, Purified
Size: 100 ug
Conjugation: FITC
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CNY 6,259.00
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Specifications
Product Data | |
Clone Name | OX-33 |
Applications | FC, IHC |
Recommend Dilution | Flow Cytometry: Use 10 µl of 1/100 diluted antibody to label 1x106 cells in 100 µl. Immunohistochemistry on Frozen and Paraffin Embedded Secions. This clone has been described reacting with paraffin-embedded material following PLP fixation (PeriodateLysine- Paraformaldehyde) (See Whiteland et al., Ref.4). |
Reactivity | Rat |
Host | Mouse |
Clonality | Monoclonal |
Immunogen | Purified rat spleen leucocyte common antigen. Spleen cells from immunised BALB/c mice were fused with cells of the NSO/U myeloma cell line. |
Specificity | SM279P is directed against a high molecular weight band of the leucocyte common antigen (LCA). OX-33 only labels B-cells among thoracic duct lymphocytes with little labelling in the bone marrow and none on thymocytes (see Ref. 2). |
Formulation | PBS, pH 7.2 containing 0.09% Sodium Azide as preservative State: Purified State: Liquid purified IgG fraction |
Concentration | lot specific |
Purification | Affinity Chromatography on Protein G |
Conjugation | Unconjugated |
Storage Condition | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
Gene Name | protein tyrosine phosphatase, receptor type, C |
Database Link | |
Background | CD45 is a complex molecule, and is comprised of different glycoproteins ranging from 180-240 kDa. Expression of CD45 is found on all hemopoietic cells. Detection of the different isoforms can distinguish between different cell forms i.e. naive T cells and memory T cells. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. The functional activity of each CD45 isotype also varies. Antibodies recognising a common epitope on all of these isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc. |
Synonyms | PTPRC, Leukocyte common antigen, L-CA, T200 |
Reference Data |
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