CD71 / TFRC Mouse Monoclonal Antibody [Clone ID: OX-26]

Specifications

Product Data
Clone Name	OX-26
Applications	FC, IHC, IP
Recommend Dilution	Flow cytometry. Immunoprecipitation. Immunohistochemistry on frozen sections. This antibody does not block the binding of transferrin to the receptor.
Reactivity	Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	PHA activated lymph node cells of a PVG rat
Specificity	This antibody recognizes the transferrin receptor. Results of flow cytometry analysis (Tissue distribution): Rat Strain: Fischer Cell concentration : 1x10e6 cells per test Antibody concentration used: 0.5 μg/10e6 cells Isotypic control: Mouse IgG2a Cell source percentage of cells stained above control: Bone Marrow 20.5% T Cell Blasts 92.2% (see picture below) Spleen 12.5%
Formulation	PBS with 0.02% sodium azide as preservative State: Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Purification	Protein G affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Database Link	UniProt ID Q99376
Background	Transferrin receptor, a 95kDa molecule is found on proliferating cells and brain endothelium.
Synonyms	TfR1, p90, Transferrin receptor protein 1
Note	Protocol: FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 μl of this suspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test). 4. To each tube, add 0.5 μg of SM289P . 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 μl of secondary antibody (FITC Goat anti-mouse IgG (H+L)). 9. Incubate the tubes at 4°C for 30-60 minutes. (It is recommended that the tubes are protected from light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C in media B. 11. Resuspend the cell pellet in 50 μl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 μl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 μl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 μl of 2M sodium azide in 100 mls).
Reference Data

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