CD11b (ITGAM) Mouse Monoclonal Antibody [Clone ID: MEM-174]

Specifications

Product Data
Clone Name	MEM-174
Applications	FC, FN, IP
Recommend Dilution	Flow Cytometry: Positive control: Peripheral Blood Lymphocytes. Immunoprecipitation: Positive control: Granulocytes CD11b/CD18 transfectants; Application note: Immunoprecipitation with the antibody was successfuly performed using immuno-affinity sorbents (minicolumns). The final immunosorbent should contain 1-5 mg of antibody/1 ml of sorbent gel (e.g. agarose beads); It is recommended to stimulate positive control material by LPS 1-2 days before to achieve better expression of target antigen). Functional Application (antibody blocks binding of LFA-1 complex to ICAM-1).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human granulocytes
Specificity	The antibody recognizes CD11b antigen (Mac-1), a 165 kDa type Itransmembrane protein mainly expressed on monocytes, granulocytes and NK-cells. The CD11b mediates neutrophil and monocyte interactions with stimulated endothelium.
Formulation	Azide free phosphate buffered saline (PBS), preserved by filter sterilization, approx. pH 7.4 State: Azide Free State: Liquid Ig fraction
Concentration	lot specific
Purification	Protein A affinity chromatography; purity: > 95% (by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Gene Name	integrin subunit alpha M
Database Link	Entrez Gene 3684 Human UniProt ID P11215
Background	CD11b (integrin aM subunit) is a 165 kDa type I transmembrane glycoprotein that non-covalently associates with integrin b2 subunit (CD18); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.
Synonyms	ITGAM, CR3A, CR-3 alpha chain, Integrin alpha-M, MAC1
Reference Data

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