CD14 Mouse Monoclonal Antibody [Clone ID: MEM-18]

Specifications

Product Data
Clone Name	MEM-18
Applications	FC, WB
Recommend Dilution	Suitable for Flow Cytometry analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity	Human, Monkey
Host	Mouse
Clonality	Monoclonal
Immunogen	A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria
Specificity	The antibody MEM-18 reacts with CD14, a 53-55 kDa GPI (glycosylphosphatidylinositol)-linked membrane glycoprotein expressed on monocytes, macrophages and weakly on granulocytes; also expressed by most tissue macrophages. In human, the epitope recognized by MEM-18 is located between amino acids 57-64.
Formulation	Phosphate buffered saline (PBS) solution containing 15mM sodium azide Label: APC State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE) Label: Conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
Conjugation	APC
Storage Condition	Store the antibody in the dark at 2-8°C. Do Not freeze! Avoid prolonged exposure to light.
Gene Name	CD14 molecule
Database Link	Entrez Gene 929 Human UniProt ID P08571
Background	CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where serves as a multifunctional lipopolysaccharide receptor; it is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipo-polysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipo-polysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes.
Synonyms	CD14
Reference Data

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