CD16 (FCGR3A) Mouse Monoclonal Antibody [Clone ID: MEM-154]

Specifications

Product Data
Clone Name	MEM-154
Applications	FC
Recommend Dilution	Flow Cytometry: Analysis of Human blood cells using 20 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human granulocytes
Specificity	This antibody reacts with the epitope on CD16 antigen that residing in proximity to FG loop (probably BC or C'E loop). CD16 is a low affinity receptor for aggregated IgG (FcgRIII antigen). The antibody reacts with CD16+ granulocytes.
Formulation	Phosphate buffered saline (PBS) containing 15 mM Sodium Azide as presrevative and 0.2% (w/v) high-grade Protease free BSA as a stabilizing agent. Label: PE State: Liquid purified IgG fraction Label: Conjugated with R-Phycoerythrin under optimum conditions
Purification	Size-Exclusion Chromatography.
Conjugation	PE
Storage Condition	Store the antibody at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Gene Name	Fc fragment of IgG receptor IIIa
Database Link	Entrez Gene 2214 Human UniProt ID P08637
Background	CD16 (FcgRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgRIII is expressed in two forms FcgRIII-A and -B. FcgRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FceRI-g subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgRIII-A is associated, moreover, with FceRI-b subunit. Besides IgG, FceRI-A can be triggered also by oligomeric IgE. FcgRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.
Synonyms	FCGR3A, CD16A, FCG3, FCGR3, IGFR3, Fc-gamma RIII-alpha, Fc-gamma RIII, Fc-gamma RIIIa, FcRIII, FcRIIIa, FcR-10, IgG Fc receptor III-2
Reference Data

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