CD34 Class III Mouse Monoclonal Antibody [Clone ID: 4H11]

Specifications

Product Data
Clone Name	4H11
Applications	FC
Recommend Dilution	Flow Cytometry analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia
Specificity	This antibody reacts with Class III epitope on CD34 (Mucosialin), a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells; it is gradually lost on progenitor cells. The antibody 4H11 [APG] completely blocks binding of Class II antibody QBEnd10 and Class III antibodies BIRMA K3 and 8G12 on KG1a cell line.
Formulation	Phosphate buffered saline (PBS) Label: APC State: Liquid Ig fraction Preservative: 15 mM Sodium Azide Label: Conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
Conjugation	APC
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Gene Name	CD34 molecule
Database Link	Entrez Gene 947 Human UniProt ID P28906
Background	CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.
Synonyms	Hematopoietic progenitor cell marker
Reference Data

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