CD4 (N-term) Mouse Monoclonal Antibody [Clone ID: MEM-241]

Specifications

Product Data
Clone Name	MEM-241
Applications	FC
Recommend Dilution	Flow Cytometry analysis of human blood cells using 20 µl reagent / 100 µl whole blood or 106 cells in a suspension.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	2 N-terminal domains of human CD4 fused to human IgG1 Fc
Specificity	The antibody reacts with CD4 antigen, a transmembrane glycoprotein (59 kDa) of the immunoglobulin supergene family present on subset of T lymphocytes ("helper/inducer" T cells) and also expressed at a lower level on monocytes and granulocytes.
Formulation	Phosphate buffered saline (PBS) containing 15 mM sodium azide Label: PE State: Liquid purified Ig fraction Label: Conjugated with R-Phycoerythrin under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
Conjugation	PE
Storage Condition	Store the antibody at 2 - 8 °C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Gene Name	CD4 molecule
Database Link	Entrez Gene 920 Human UniProt ID P01730
Background	CD4 is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1); HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1); IL-16 (binds to CD4 domain 3), Human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin. Intracellular ligands: p56Lck. CD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus; CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
Synonyms	T-cell surface antigen T4/Leu-3
Reference Data

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