CD45 (PTPRC) Mouse Monoclonal Antibody [Clone ID: MEM-28]

Specifications

Product Data
Clone Name	MEM-28
Applications	FC
Recommend Dilution	Immunofluorescence analysis by Flow Cytometry: 1: 500 as starting point.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human thymocytes and T lymphocytes
Specificity	The antibody reacts with all alternative forms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets.
Formulation	Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 Label: Biotin State: Liquid Ig fraction Label: Conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
Concentration	lot specific
Conjugation	Biotin
Storage Condition	Store the antibody undiluted at 2 - 8 °C. DO NOT FREEZE!
Gene Name	protein tyrosine phosphatase, receptor type C
Database Link	Entrez Gene 5788 Human UniProt ID P08575
Background	CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.
Synonyms	PTPRC, Leukocyte common antigen, L-CA, T200
Reference Data

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