CD71 (TFRC) Mouse Monoclonal Antibody [Clone ID: MEM-189]

Specifications

Product Data
Clone Name	MEM-189
Applications	FC, IP, WB
Recommend Dilution	Flow Cytometry: 2 µg/ml. Immunoprecipitation. Western Blot (Non Reducing Conditions).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	KG1 human acute myelogenous leukaemia cell line
Specificity	This antibody reacts with CD71 antigen (transferrin receptor), a 95 kDa type II homodimeric transmembrane glycoprotein expressed on activated B and lymphocytes, macrophages and erythroid precursors; it is lost on resting blood leukocytes. It does not block binding of transferrin to the receptor.
Formulation	TBS buffer pH 8.0 State: Purified State: Liquid purified IgG fraction (> 95% by SDS-PAGE) Preservative: 15 mM Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	transferrin receptor
Database Link	Entrez Gene 7037 Human UniProt ID P02786
Background	CD71 (transferrin receptor) is a type II transmembrane glycoprotein expressed as homodimer in erythroid blood cell line and in activated leukocytes. Upon binding of holotransferrin (complex of transferrin and iron ions), CD71 is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with CD71 and recycles to the cell surface, where it is released upon exposure to normal pH. CD71 is also involved in uptake of non-transferrin bound iron.
Synonyms	TfR1, p90, Transferrin receptor protein 1
Reference Data

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