Cytokeratin 18 (KRT18) Mouse Monoclonal Antibody [Clone ID: DA-7]

Specifications

Product Data
Clone Name	DA-7
Applications	ELISA, IF, IHC, IP, WB
Recommend Dilution	Immunoprecipitation. Western Blot: 1-2 µg/ml overnight in 4°C. Sample Preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail); Incubate 60 min on ice; Centrifuge to remove cell debris; Mix lysate with non-reducing SDS-PAGE sample buffer. Positive Control: HeLa human cervix carcinoma cell line, A-431 human epidermoid carcinoma cell line, MCF-7 human breast adenocarcinoma cell line. Immunohistochemistry on Paraffin Sections: 10 µg/ml. Positive Tissue: Epithelial tissue (skin), Colon. Immunocytochemistry. Positive Control: MCF-7 Human breast adenocarcinoma cell line, HeLa human cervix carcinoma cell line. ELISA: The antibody DA-7 has been tested as the detection antibody in a Sandwich ELISA for analysis of Cytokeratin 18 in combination with the antibody C-04 (Cat.-No SM3043P). Positive Control: MCF-7 human breast adenocarcinoma cell line.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human breast carcinoma cell line PMC-42.
Specificity	The antibody DA-7 reacts with Cytokeratin 18 (45 kDa). Negative Species: Porcine, Mouse, Rat, Hamster, Bovine, Sheep, Canine (Dog).
Formulation	PBS, pH~7.4 State: Purified State: Liquid purified IgG fraction (> 95% by SDS-PAGE) Preservative: 15 mM Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	keratin 18
Database Link	Entrez Gene 3875 Human UniProt ID P05783
Background	Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues. Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. Cytokeratins 18 belongs to type I family (acidic cytokeratins).
Synonyms	Cytokeratin-18, CK18, Keratin-18, Keratin 18, KRT18, CYK18, K18
Reference Data

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