Neurofilament M (160 kD) Mouse Monoclonal Antibody [Clone ID: NF-09]

Specifications

Product Data
Clone Name	NF-09
Applications	IF, IHC, WB
Recommend Dilution	Western blot. Immunocytochemistry. Immunohistochemistry on Paraffin Sections. Positive Tissue: Neuro2A murine cell line. Note: Carnoys fixative 2 x 3 min, blocking 1% Glycine + 0.2% Gelatin 10 min.
Reactivity	All Species
Host	Mouse
Clonality	Monoclonal
Immunogen	Pellet of Porcine brain cold stable proteins after depolymerization of microtubules
Specificity	The antibody reacts with both phosphorylated and non-phosphorylated form of medium neurofilament protein (160 kDa) of various species. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.
Formulation	Phosphate buffered saline (PBS), pH~7.4 with 15 mM Sodium Azide as preservative. State: Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Database Link	UniProt ID P08552
Background	Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons, the longest cell protrusions known in vertebrates. NFs in most vertebrates are composed of three different polypeptide chains with different molecular weights (NF-H - heavy chain, NF-M - medium chain and NF-L - light chain). The three NF subunits share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm.
Synonyms	Neurofilament medium polypeptide, NF-M, NEF3, NEFM, Neurofilament 3, (Neuronal Marker)
Reference Data

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