CD38 Mouse Monoclonal Antibody [Clone ID: HIT2]

Specifications

Product Data
Clone Name	HIT2
Applications	FC, IHC, WB
Recommend Dilution	Flow Cytometry. Western Blot (Non Reducing Conditions): 2 µg/ml. Positive Control: RAJI Human cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Boil for 5 min. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: 10 µg/ml.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human thymocytes in foetus
Specificity	The antibody reacts with CD38 (T10), a 45 kDa type II transmembrane glycoprotein strongly expressed mainly on plasma cells and activated T and B lymphocytes; it is an antigenic marker of lymphoid cells.
Formulation	PBS, pH~7.4 State: Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE) Preservative: 15mM Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	CD38 molecule
Database Link	Entrez Gene 952 Human UniProt ID P28907
Background	CD38 (NAD+ glycohydrolase) is a type II transmembrane glycoprotein able to induce activation, proliferation and differentiation of mature lymphocytes and mediate apoptosis of myeloid and lymphoid progenitor cells. Another role of CD38 is provided by enzymatic activity of its extracellular part. CD38 acts as NAD+ glycohydrolase converting NAD+ into ADP-ribose, as ADP-ribosyl cyclase producing cADPR and as cADPR hydrolase, thus affecting levels of calcium-mobilizing metabolites. ADPR produced by CD38 serves as an important second messenger of neutrophil and dendritic cell migration.
Synonyms	cADPr hydrolase 1, T10
Reference Data

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