CD18 (ITGB2) Mouse Monoclonal Antibody [Clone ID: MEM-48]

Specifications

Product Data
Clone Name	MEM-48
Applications	FC, IHC, WB
Recommend Dilution	Flow Cytometry analysis of human blood cells using 20 µl reagent / 100 µl whole blood.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Leucocytes of a patient suffering from a LGL-type leukaemia
Specificity	The antibody recognizes an epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen (Integrin beta2 chain).
Formulation	Phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent Label: FITC State: Liquid purified Ig fraction Label: Conjugated with Fluorescein isothiocyanate under optimum conditions. The reagent is free of unconjugated and adjusted for direct use
Conjugation	FITC
Storage Condition	Store the antibody at 2 - 8 °C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Gene Name	integrin subunit beta 2
Database Link	Entrez Gene 3689 Human UniProt ID P05107
Background	CD18 is a 90-95 kDa type I transmembrane protein expressed on all leukocytes. CD18, integrin b2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (b2) integrins: aLb2 (CD11a/CD18, LFA-1), aMb2 (CD11b/CD18, Mac-1, CR3), aXb2 (CD11c/CD18) and aDb2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.
Synonyms	Integrin beta-2, MFI7
Reference Data

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