FYN Mouse Monoclonal Antibody [Clone ID: FYN-01]

Specifications

Product Data
Clone Name	FYN-01
Applications	IF, IHC, IP, WB
Recommend Dilution	Immunoprecipitation: Positive control: J77 T cell line. Preparation of cell lysate: 30 min on ice (orbital incubator) in lysing buffer; lysing buffer with N-dodecyl beta-D-maltoside (20 mM Tris/Cl, 100 mM NaCl pH 8,2, 1% laurylmaltosid (w/v), 50 mM NaF). Application note: The antibody FYN-01 apparently gives very specific signal (by 59 kDa), and nicely immunoprecipitates Fyn from cell lysates.) Western Blotting: 2 µg/ml Positive control: JURKAT human T cell leukemia cell line. Immunocytochemistry. Immunohistochemistry on Paraffin Sections: 5 μg/ml. Positive tissue: brain.
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Bacterially expressed recombinant fragment of human Fyn (aa 7-176)
Specificity	The antibody reacts with p59fyn (Fyn), a non receptor protein tyrosine kinase (~59 kDa), which belongs to the Src family of cytoplasmic tyrosine kinases.
Formulation	Phosphate buffered saline (PBS) with 15 mM Sodium Azide, approx. pH 7.4 State: Purified State: Liquid purified Ig fraction (> 95% by SDS-PAGE)
Concentration	lot specific
Purification	Protein A Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Gene Name	FYN proto-oncogene, Src family tyrosine kinase
Database Link	Entrez Gene 2534 Human UniProt ID P06241
Background	Fyn is a membrane-associated, non-receptor protein tyrosine kinase approximately 59kDa, which belongs to the Src family of cytoplasmic tyrosine kinases. Fyn is very strongly similar to mouse Fyn, v-yes and c-src. Fyn is expressed predominately in tissues of neuronal and hematopoietic origin. Neuronal Fyn and hematopoietic Fyn differ at the junction of the SH2 and kinase domains due to tissue specific alternative splicing. Fyn has been shown to be involved in B cell and T cell activation as well as keratinocyte differentiation. In T cells, Fyn associates with the T-cell antigen receptor and Thy1.
Synonyms	p59-Fyn, Protooncogene Syn, SLK
Reference Data

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