KIF5B Mouse Monoclonal Antibody [Clone ID: KN-02]

Specifications

Product Data
Clone Name	KN-02
Applications	IF
Recommend Dilution	Immunocytochemistry.
Reactivity	Human, Porcine, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Enriched fraction of porcine brain kinesin.
Specificity	The antibody KN-02 recognizes heavy chain of conventional kinesin associated with vesicles and with lower affinity with denaturated molecule. Epitope is located in coiled-coil stalk domain. It stains Western blots of kinesin-enriched preparations. Epitope mapping (by limited proteolysis of partially purified porcine kinesin) followed by immunoblotting has revealed that antibodies KN-01, KN-02 and KN-03 react with different sets of fragments. The antibody KN-02 does not react with kinesin bound to taxol-stabilized microtubules.
Formulation	Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 State: Purified State: Liquid Ig fraction
Concentration	lot specific
Purification	Thiophilic adsorption-affinity chromatography; purity: > 95 % (by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Gene Name	kinesin family member 5B
Database Link	Entrez Gene 3799 Human UniProt ID P33176
Background	Kinesin belongs to the group of microtubule-associated motor proteins known to convert chemical energy released from nucleoside triphosphates (preferentially from ATP) into mechanical energy. Conventional kinesin, member of the kinesin superfamily comprising more than 100 proteins, is involved in the anterograde vesicle transport in neuronal cells. Kinesin purified from mammalian brain homogenates is a heterotetramer consisting of two heavy (120 to 130 kDa) and two light chains (60 to 70 kDa), resulting in a molecular mass about 400 kDa. Each heavy chain contains an N-terminal globular motordomain with both a microtubule-binding site and an ATPase active center, stalk region responsible for heavy chain dimerization and finally C-terminal globular tail domain, which is implicated in cargo binding. Light chains may have a regulatory function.
Synonyms	KIF5B, KNS, KNS1, Kinesin-1 heavy chain, Ubiquitous kinesin heavy chain, UKHC, Conventional kinesin heavy chain
Reference Data

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