Vimentin (VIM) Mouse Monoclonal Antibody [Clone ID: VI-01]

Specifications

Product Data
Clone Name	VI-01
Applications	FC, IF, WB
Recommend Dilution	Western blot: 1-2 μg/ml. Immunocytochemistry: Staining technique: RBL Rat basophilic leukemia cell line: (a) Fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C; (b) Fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C. Incubation Time: 45 min RT Positive Control: 3T3 Mouse Swiss albino fibroblast cell line RBL, Rat basophilic leukemia cell line.
Reactivity	All Species
Host	Mouse
Clonality	Monoclonal
Immunogen	Pellet of pig brain cold stable proteins after depolymerization of microtubules
Specificity	The antibody reacts with Vimentin, a 57 kDa intermediate filament expressed in variety of mesenchymal and mesodermal cell types in all species (recognized epitope conserved within all species). Cross-reactivity was found with smooth muscle Desmin.
Formulation	PBS , pH~7.4 State: Purified State: Liquid purified Ig fraction (> 95% by SDS-PAGE) Preservative: 15 mM Sodium Azide
Concentration	lot specific
Purification	Precipitation Methods
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2 - 8°C. DO NOT FREEZE!
Gene Name	vimentin
Database Link	UniProt ID P08670
Background	Vimentin (57 kDa) is the most ubiquituos intermediate filament protein and the first to be expressed during cell differentiation. All primitive cell types express vimentin but in most non-mesenchymal cells it is replaced by other intermediate filament proteins during differentiation. Vimentin is expressed in a wide variety of mesenchymal cell types - fibroblasts, endothelial cells etc., and in a number of other cell types derived from mesoderm, e.g., mesothelium and ovarian granulosa cells. In non-vascular smooth muscle cellsand striated muscle, vimentin is often replaced by desmin, however, during regeneration, vimentin is reexpressed. Cells of the lymfo-haemopoietic system (lymphocytes, macrophages etc.) also express vimentin, sometimes in scarce amounts. Vimentin is also found in mesoderm derived epithelia, e.g. kidney (Bowman capsule), endometrium and ovary (surface epithelium), in myoepithelial cells (breast, salivary and sweat glands), an in thyroid gland epithelium. In these cell types, as in mesothelial cells, vimentin is coexpressed with cytokeratin. Furthermore, vimentin is detected in many cells from the neural crest. Particularly melanocytes express abundant vimentin. In glial cells vimentin is coexpressed with Glial Fibrillary Acidic Protein (GFAP). Vimentin is present in many different neoplasms but is particulary expressed in those originated from mesenchymal cells. Sarcomas e.g., fibrosarcoma, malignt fibrous histiocytoma, angiosarcoma, and leio- and rhabdomyosarcoma, as well as lymphomas, malignant melanoma and schwannoma, are virtually always vimentin positive. Mesoderm derived carcinomas like renal cell carcinoma, adrenal cortical carcinoma and adenocarcinomas from endometrium and ovary usually express vimentin. Also thyroid carcinomas are vimentin positive. Any low differentiated carcinoma may express some vimentin. Vimentin is frequently included in the so-called primary panel (together with CD45, cytokeratin, and S-100 protein). Intense staining reaction for vimentin without coexpression of other intermediate filament proteins is strongly suggestive of a mesenchymal tumour or malignant melanoma.
Synonyms	VIM
Reference Data

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