CSK Mouse Monoclonal Antibody [Clone ID: CSK-04]

Specifications

Product Data
Clone Name	CSK-04
Applications	IF, IP, WB
Recommend Dilution	Immunoprecipitation. Western Blotting (1:250-1:300. Weak non-specific band of cca 60 kDa is detected in human cell lines in lower antibody dilutions). Immunochemistry (Positive control: HeLa human cervix carcinoma cell line).
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Bacterially expressed recombinant fragment of human Csk corresponding to aa 330-450.
Specificity	The antibody CSK-04 reacts with Csk (C-terminal Src kinase), a 50 kDa non-receptor protein tyrosine kinase expressed in cytosol.
Formulation	Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 State: Purified State: Liquid Ig fraction
Purification	Protein A affinity chromatography; purity: > 95% (by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Gene Name	c-src tyrosine kinase
Database Link	Entrez Gene 1445 Human UniProt ID P41240
Background	C-terminal Src kinase (Csk) is a non-receptor protein tyrosine kinase which resembles Src-family kinases, but unlike them lacks the conserved autophosphorylation site, the regulatory C-terminal tyrosine as well as myristylation and palmitylation. Csk negatively regulates Src-family kinases by phosphorylation of their C-terminal regulatory tyrosine. Disruption of the csk gene causes constitutive activation of Src-family kinases, and overexpression of Csk usually counteracts their signaling. The Csk-mediated regulation of those Src-family kinases that are localized in lipid rafts is enabled by a ubiquitously expressed transmembrane adaptor PAG (also known as Cbp, Csk-binding protein), which recruits Csk.
Synonyms	Tyrosine-protein kinase CSK, C-SRC kinase, Protein-tyrosine kinase CYL
Reference Data

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