Glycophorin A (GYPA) (N-term) Mouse Monoclonal Antibody [Clone ID: HIR2]

Specifications

Product Data
Clone Name	HIR2
Applications	AGG, FC, IHC
Recommend Dilution	Flow Cytometry: This HIR2 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes and cell agglutination assay and can be used at approximately 0.1 µg per million cells. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Embedded Sections. Agglutination: Clone HIR2 agglutinates untreated RBCs but it failes to agglutinate papain-treated cells.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptide (Human, N-terminal)
Specificity	The mouse monoclonal antibody HIR2 recognizes an epitope within the N-terminal (extracellular) portion of CD235a (glycophorin A) and CD235b (glycophorin B). Its antigen is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells.
Formulation	PBS, pH~7.4 State: Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE) Preservative: 15 mM Sodium Azide
Concentration	lot specific
Purification	Protein A Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	glycophorin A (MNS blood group)
Database Link	Entrez Gene 2993 Human UniProt ID P02724
Background	CD235a (Glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors. Similarly to glycophorin B (GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between erythrocytes in the circulation. GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. CD235a is a receptor of Hsa, an Streptococcus adhesin.
Synonyms	Glycophorin-A, GPA, PAS-2, Sialoglycoprotein alpha, MN sialoglycoprotein
Reference Data

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