GST-Tag Mouse Monoclonal Antibody [Clone ID: S-tag-05]

Specifications

Product Data
Clone Name	S-tag-05
Applications	IP, WB
Recommend Dilution	Western blot: 1 µg/ml (Reducing Conditions). Positive Control: GST fusion protein. Immunoprecipitation.
Reactivity	Escherichia coli, Mammalian, Schistosoma japonicum
Host	Mouse
Clonality	Monoclonal
Immunogen	GST fusion protein.
Specificity	The GST-Tag monoclonal antibody S-tag-05 detects Glutathione-S-Transferase (GST) from Schistosoma japonicum. It is suitable for detecting fusion proteins containing the GST-Tag sequence expressed in E. coli, Yeast, Mammalian, and in vitro transcription/translation systems. Recognizes fusion proteins in all Species.
Formulation	PBS, pH 7.4 with 0,05% Sodium Azide as preservative State: Purified State: Liquid purified IgG fraction (>95% pure by SDS-PAGE)
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Database Link	UniProt ID P08515
Background	The GST (Glutathione-S-Transferase) is a 26kDa protein encoded by the parasite Schistosoma japonicum, easily purified by affinity chromatography on immobilized glutathione. GST is utilized as a fusion protein with foreign proteins in a range of prokaryotic expression vectors, including the pGEX family of vectors. Antibodies against GST will detect the expressed GST protein or a GST-fusion protein, and can be utilized for immuno-purification of GST-fusion proteins.
Synonyms	Glutathione S-transferase Tag, GST26-Tag
Reference Data

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