Thy1 (Thy1.2) Mouse Monoclonal Antibody [Clone ID: 1aG4/C5]

Specifications

Product Data
Clone Name	1aG4/C5
Applications	FC, FN, IF, WB
Recommend Dilution	Flow Cytometry: 1 µg/ml Western Blotting.  Immunocytochemistry. Functional Assay: The bound of antibody 1aG4/C5 to the transfected mast cells induces the activation processes which are similar, but not identical, to the antigen activation of IgE stimulated cells. Other studies approve that the antibody 1aG4/C5 precipitates complexes with Lyn protein tyrosine kinase.
Reactivity	Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Thymocytes from C3H/Di mice (alelic form: Thy-1.2 positive)
Specificity	The antibody 1aG4/C5 recognizes alloantigen Thy-1.2 of Mouse Thy-1 (CD90). It does not cross-react with Thy-1.1 alloantigen. Thy-1 is a 18 kDa cell surface GPI linked glycoprotein (Immunoglobulin superfamily); in the mouse it is expressed on all thymocytes and peripheral T lymphocytes.  Negative Species: Human, Rat.
Formulation	Azide free PBS, pH~7.4, sterilized by filteration State: Azide Free State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE) Preservative: None
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	thymus cell antigen 1, theta
Database Link	Entrez Gene 21838 Mouse UniProt ID P01831
Background	CD90 (Thy-1) is an 18-35 kDa GPI-anchored plasma membrane glycoprotein expressed in many cell types, such as in hematopoietic cells and neurons, connective tissues, various fibroblast and stromal cell lines, tumor endothelial cell lines and other. In the mouse, CD90 is expressed mainly on thymocytes and peripheral T lymphocytes. It is involved in T cell activation, cellular adhesion, proliferation and migration, neurite outgrowth, wound healing, apoptosis, and fibrosis. CD90 participates in multiple signaling cascades and its effects are tissueand cell type-specific. It often functions as an important regulator of cell-cell and cell-matrix interactions.
Synonyms	Thy-1, THY1, CDw90
Reference Data

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