CD5 Rat Monoclonal Antibody [Clone ID: YKIX322.3]

Specifications

Product Data
Clone Name	YKIX322.3
Applications	FC
Recommend Dilution	Flow Cytometry: Neat - 1/10; Use 10µl of the suggested working dilution to label 10e6 cells or 100µl of whole blood.
Reactivity	Canine
Host	Rat
Clonality	Monoclonal
Immunogen	Concanavilin A activated canine peripheral blood cells. Spleen cells from an immunised DA rat were fused with cells of the rat Y3/Ag1.2.3 myeloma cell line.
Specificity	This antibody recognises a 67kD cell surface glycoprotein expressed on the surface of T cells. Clustered as canine CD5 in the First Canine Leucocyte Antigen Workshop.
Formulation	PBS, pH 7.4 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin Label: FITC State: Liquid purified IgG Label: Fluorescein Isothiocyanate Isomer 1
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	FITC
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Background	CD5 is a 55kDa T lymphocyte single chain transmembrane glycoprotein. It is present on all mature T lymphocytes, on most thymocytes and on many T cell leukemias and lymphomas. It reacts with a subpopulation of activated B cells. CD5/Lyt1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. It has been identified as the major ligand of the B cell antigen CD72. The frequency of CD5+ B cells exhibits strain dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B1 cells. Binding of CD5 on the T cell surface can augment alloantigen or mitogen induced lymphocyte proliferation and induces increased cytosolic free calcium, IL2 secretion, and IL2R expression. It has been proposed that CD5 negatively regulates signal transduction mediated by the T cell and B cell receptors.
Synonyms	LEU1, LEU-1, Ly-1, Lyt-1, Lymphocyte antigen T1/Leu-1, T-cell surface glycoprotein CD5
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.