CD4 Mouse Monoclonal Antibody [Clone ID: CC30]

Specifications

Product Data
Clone Name	CC30
Applications	FC, IHC, IP
Recommend Dilution	Flow Cytometry: Use 10 µl of 1/25-1/200 diluted antibody to label 106 cells in 100 µl. Immunoprecipitation. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections. Clone CC30 has been reported as being suitable for use on Formalin Dichromate (FD5) Fixed Paraffin Embedded tissue with amplification and antigen retrieval techniques (See Ref.3 for details).
Reactivity	Bovine
Host	Mouse
Clonality	Monoclonal
Immunogen	Bovine thymocytes. Spleen cells from an immunised mouse were fused with cells of the mouse NS1 myeloma cell line.
Specificity	This antibody precipitates a molecule of approximately 50kDa and is considered to be the Bovine homologue of Human CD4.
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Database Link	Entrez Gene 407098 Bovine UniProt ID A7YY52
Background	The phenotype, tissue distribution and function of T-cells expressing the bovine CD4 antigen are similar to those in other species. However, expression on macrophages has not yet been detected.
Synonyms	T-cell surface antigen T4/Leu-3
Reference Data

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