CD31 / PECAM1 Mouse Monoclonal Antibody [Clone ID: CO.3E1D4]

Specifications

Product Data
Clone Name	CO.3E1D4
Applications	FC, IP
Recommend Dilution	Immunoprecipitation. Flow Cytometry: Use 10 µl of 1/10-1/25 diluted antibody to label 1 x 106 cells in 100 µl.
Reactivity	Bovine, Goat, Sheep
Host	Mouse
Clonality	Monoclonal
Immunogen	Ovine leucocytes. Spleen cells from immunised BALB/c mice were fused with cells of the SP2-0/Ag14 mouse myeloma cell line.
Specificity	This antibody is specific for CD31, also known as PECAM-1. Clone CO.3E1D4 is reported to inhibit homotypic leucocyte aggregation induced by anti CD43 antibodies (2). Removal of Sodium Azide is recommended prior to use in Fuctional Assays.
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Database Link	UniProt ID P51866
Background	CD31, also known as platelet endothelial cell adhesion molecule 1 (PECAM1), is a type I integral membrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is constitutively expressed on the surface of endothelial cells, and concentrated at the junction between them. It is also weakly expressed on many peripheral lymphoid cells and platelets. CD31 has been used to measure angiogenesis in association with tumor recurrence. Other studies have also indicated that CD31 and CD34 can be used as markers for myeloid progenitor cells and recognize different subsets of myeloid leukemia infiltrates (granular sarcomas). Ovine CD31 is predominantly expressed by peripheral blood platelets and a small percentage of lymphocytes. CD31 is also highly expressed by ovine endothelial cells.
Synonyms	PECAM-1, EndoCAM, GPIIA'
Reference Data

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