MIF Mouse Monoclonal Antibody [Clone ID: 4E4]

Specifications

Product Data
Clone Name	4E4
Applications	ELISA, FC, IF, IHC, WB
Recommend Dilution	ELISA. Western blot: 1/500-1/2,000. Recommended starting dilution is 1/1,000. Flow Cytometry.  Immunofluorescence/Immnunocytochemistry. Immunohistochemistry on Paraffin Sections: 5 µg/ml.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Human MIF (1-115 aa) purified from E. coli.
Specificity	The antibody recognizes Human MIF. Other species not tested.
Formulation	PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol State: Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	macrophage migration inhibitory factor (glycosylation-inhibiting factor)
Database Link	Entrez Gene 4282 Human UniProt ID P14174
Background	The cytokine Macrophage migration inhibitory factor (MIF) has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T-cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration.
Synonyms	Macrophage migration inhibitory factor, Phenylpyruvate tautomerase, Glycosylation-inhibiting factor, GLIF, MMIF
Reference Data

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