Caspase-7 (CASP7) (C-term) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Western blot: 1.0-2.0 µg/ml.  Immunohistochemistry on Paraffin Sections: 2.0 µg/ml. This product does not require protein digestion pre-treatment of paraffin sections eg. Trypsin or pronase prior to staing.  This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. Recommended Positive Control: Skeletal muscle tissue.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	A 16 amino acid peptide from Human Caspase-7 carboxy-terminus
Specificity	This antibody recognises an epitope within the C-terminal region (CT) of Caspase-7, a 35kDa cysteine protease, otherwise known as ICE-like Apoptotic Protease 3 (ICE-LAP3) Caspase-7.
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	caspase 7
Database Link	Entrez Gene 840 Human UniProt ID P55210
Background	ICE-LAP3 is a member of the ICE/Ced-3 subfamily, is an executioner caspase which undergoes proteolytic cleavage of its precursor to form active p12 and p20 subunits. Evidence that activation of Caspase-7 occurs during cell death induced by the cytokine death receptors Fas/APO-1 and the receptor of tumour necrosis factor (TNFR-1), coupled with the fact that granzyme-B activated Caspase-7 cleaves the nuclear enzyme poly (ADPribose) polymerase (PARP), suggests an important role for Caspase-7 in both cytokine-mediated and Granzyme-B mediated apoptosis pathways.
Synonyms	CASP-7, CASP7, MCH3, CMH-1
Reference Data

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