Blnk Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	Western Blot: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Application note: Reducing conditions.
Reactivity	Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Fusion protein of human BLNK
Specificity	The antibody reacts with BLNK, a cytosolic protein identified as two phosphoproteins migrating at 68 and 70 kDa in SDS/PAGE (alternatively spliced forms of human BLNK).
Formulation	PBS, pH 7.4, containing 15 mM sodium azide as preservative. State: Purified State: Liquid Serum
Concentration	lot specific
Purification	Precipitation methods; purity < 95% (by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	B cell linker
Database Link	Entrez Gene 17060 Mouse UniProt ID Q9QUN3
Background	SLP65 / BLNK (SH2 domain-containing leukocyte-specific phosphoprotein of 65 kDa; B cell linker protein), also known as BASH, is an adaptor protein that plays key role in B cell activation initiated by cross-linking the B cell receptor (BCR). Phosphorylated by Syk tyrosine kinase, SLP65 serves as a scaffold for Btk tyrosine kinase, Vav1 guanine nucleotide exchange factor, phospholipase Cg2, as well as Grb2 and Nck adaptor proteins; thus represents a central linker protein that bridges the BCR-associated kinases with a multitude of signaling pathways.
Synonyms	BASH, SLP65, SLP-65
Reference Data

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