Beclin 1 (BECN1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	FC, ICC/IF, IHC, IP, Simple Western, WB
Recommend Dilution	Immunohistochemistry-Frozen: 1:400, Flow Cytometry: 2-10 ug/ml, Knockdown Validated, Simple Western: 1:50, Immunohistochemistry: 1:400, Immunocytochemistry/ Immunofluorescence: 1:50-1:200, Immunoprecipitation: 1:80, Immunohistochemistry-Paraffin: 1:400, Western Blot: 1:500-1:2000
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	NB 500-249 was raised against an internal synthetic peptide to human Beclin 1, containing residues within 1-100. This sequence has 94% identity with the mouse sequence and 88% identity with the rat sequence.
Formulation	Tris-citrate/phosphate, pH 7-8 and 0.1% sodium azide
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	beclin 1
Database Link	NP_003757 Entrez Gene 56208 MouseEntrez Gene 8678 Human UniProt ID Q14457
Background	Beclin 1 is the first identified mammalian gene to mediate autophagy and also has tumor suppressor and antiviral function. Autophagy, a process of bulk protein degradation through an autophagosomic-lysosomal pathway, is important for differentiation, survival during nutrient deprivation, and normal growth control, and is often defective in tumor cells. Beclin 1 was originally isolated in a yeast two hybrid screen to identify Bcl-2-binding partners and maps to a tumor susceptibility locus on human chromosome 17q21 that is frequently monoallelically deleted in human breast, ovarian and prostate cancer. Beclin 1 encodes an evolutionarily conserved 60 kDa coiled coil protein that is expressed in human muscle, epithelial cells and neurons. In gene transfer studies, beclin 1 promotes nutrient deprivation-induced autophagy, inhibits mammary tumorigenesis, and inhibits viral replication. Expression of the Beclin 1 protein is frequently decreased in malignant breast epithelial cells. Based upon these observations, it is speculated that beclin 1 may work through induction of autophagy to negatively regulate tumorigenesis and to control viral infections. Beclin 1 may also play a role in other biological processes in which autophagy is important such as cell differentiation and nutritional stress responses.
Synonyms	ATG6; beclin1; VPS30
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Regulation of autophagy

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.