ATG5 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	Electron Microscopy, ELISA, FC, ICC/IF, IHC, Immunoblotting, IP, Simple Western, WB
Recommend Dilution	Radioimmunoassay, ELISA, Immunoblotting, Knockout Validated, Knockdown Validated, Electron Microscopy, Flow Cytometry, Western Blot: 1:500, Simple Western: 1:50, Immunocytochemistry/ Immunofluorescence: 1:250, Immunohistochemistry: 1:400, Immunohistochemistry-Paraffin: 1:400, Immunoprecipitation: 1:10 - 1:500, Proximity Ligation Assay
Reactivity	Human, Mouse, Porcine, Primate, Xenopus, Zebrafish, Cow, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide made to an N-terminal region (within residues 1-50) of the human ATG5 protein. [Swiss-Prot# Q9H1Y0]
Formulation	PBS plus 30% glycerol and 0.1% sodium azide
Concentration	lot specific
Purification	peptide affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	autophagy related 5
Database Link	NP_004840 Entrez Gene 11793 MouseEntrez Gene 365601 RatEntrez Gene 9474 Human UniProt ID Q9H1Y0
Background	Autophagy is an important mechanism for nonselective intracellular breakdown whereby cytosol and organelles are encapsulated in vesicles, which are then engulfed and digested by lytic vacuoles/lysosomes. ATG5 is required for autophagy. It conjugates to ATG12 and associates with isolation membrane to form autophagosomes. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. Conjugation to ATG12 is essential for autophagy, but is not required for association with isolation membrane. ATG5 also plays an important role in the apoptotic process. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.
Synonyms	APG5; APG5-LIKE; APG5L; ASP; hAPG5
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Regulation of autophagy, RIG-I-like receptor signaling pathway

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