BubR1 (BUB1B) Mouse Monoclonal Antibody [Clone ID: 8G1]

Specifications

Product Data
Clone Name	8G1
Applications	CyTOF-ready, FC, ICC/IF, IP, WB
Recommend Dilution	Flow Cytometry: 1 ug per million cells, Immunoprecipitation: 1:10-1:500, Western Blot: 1:2000-1:5000, Immunocytochemistry/ Immunofluorescence: 1:100, CyTOF-ready
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	A portion of hBUBR1 tagged at the N-terminus with pMAL (residues 1-350 of human BUBR1).
Formulation	PBS with 0.1% Sodium Azide
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	119.4 kDa
Gene Name	BUB1 mitotic checkpoint serine/threonine kinase B
Database Link	NP_001202 Entrez Gene 12236 MouseEntrez Gene 701 Human UniProt ID O60566
Background	Compromised spindle checkpoints are thought to play an important role in genetic instability that predisposes cells to malignant transformations. Human cells contain two related checkpoint protein kinases, evolved from the BUB1 gene; hBUBR1 and hBUB1. They are important components of the spindle checkpoint Both kinases monitor mitotic kinetochore-microtubule interactions. hBUBR1 is essential for normal mitotic progression as it prevents cells from entering into anaphase too early. BUBR1 has been detected in human cancers. It has been identified as a novel binding partner of BCSG1 (Breast Cancer-Specific Gene 1), which has been suggested to accelerate the progression of breast cancer by compromising the mitotic checkpoint control through inactivation of BUBR1.
Synonyms	Bub1A; BUB1beta; BUBR1; hBUBR1; MAD3L; MVA1; SSK1
Reference Data
Protein Families	Druggable Genome, Protein Kinase, Stem cell - Pluripotency
Protein Pathways	Cell cycle

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