ASK1 (MAP3K5) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, ICC, IF, WB
Recommend Dilution	ASK1 antibody can be used for detection of ASK1 by Western blot at 0.5 µg/mL. A 155 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 10 µg/mL. For immunofluorescence start at 20 µg/mL. Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	ASK1 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human ASK1 This sequence is different from that of mouse by last two amino acids.
Formulation	PBS containing 0.02% sodium azide.
Concentration	1ug/ul
Purification	ASK1 Antibody is DEAE purified.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	mitogen-activated protein kinase kinase kinase 5
Database Link	NP_005914 Entrez Gene 4217 Human UniProt ID Q99683
Background	Mitogen-activated protein (MAP) kinase cascades are activated in response to various extracellular stimuli, including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was recently identified and designated ASK1 (for apoptosis signal-regulating kinase 1) and MAPKKK5. ASK1 activated two different subgroups of MAPKK, MKK4 and MKK6, which in turn activated c-Jun N-terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1/MAPKKK5 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas-associated protein Daxx. Overexpression of ASK1 induced apoptotic cell death, and a catalytically inactive form of ASK1 inhibited TNF-alpha-induced apoptosis. ASK1 is expressed in variety of human and mouse tissues.
Synonyms	ASK1; MAPKKK5; MEKK5
Reference Data
Protein Families	Druggable Genome, Protein Kinase
Protein Pathways	Amyotrophic lateral sclerosis (ALS), MAPK signaling pathway, Neurotrophin signaling pathway

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