CXCR4 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, ICC, IF, WB
Recommend Dilution	CXCR4-Lo antibody can be used for Western blot at 10 µg/mL. Antibody can also be used for immunocytochemistry starting at 2 µg/mL. For immunofluorescence start at 4 µg/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	CXCR4-Lo antibody was raised against a peptide corresponding to nine amino acids near the amino terminus of human CXCR4 isoform a.
Specificity	This antibody is specific for the longer isoform of CXCR4.
Formulation	PBS containing 0.02% sodium azide.
Concentration	1ug/ul
Purification	CXCR4-Lo Antibody is affinity chromatography purified via peptide column.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	C-X-C motif chemokine receptor 4
Database Link	NP_001008540 Entrez Gene 7852 Human UniProt ID P61073
Background	Human immunodeficiency virus (HIV) and related viruses require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors. Among them, CXCR4 is a principal coreceptor for T-cell tropic strains of HIV-1 fusion and entry of human white blood cells. CXCR4 is also required for the infection by dual-tropic strains of HIV-1 and mediates CD-4 independent infection by HIV-2. The a-chemokine SDF-1 is the ligand for CXCR4 and prevents infection by T-tropic HIV-1. CXCR4 associates with the surface CD4-gp120 complex before HIV enters target cells. CXCR4 messenger RNA levels correlated with HIV-1 permissiveness in diverse human cell types. Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells. The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV binding sites. This antibody is specific for the longer isoform of CXCR4.
Synonyms	CD184; D2S201E; FB22; HM89; HSY3RR; LAP-3; LAP3; LCR1; LESTR; NPY3R; NPYR; NPYRL; NPYY3R; WHIM
Reference Data
Protein Families	Druggable Genome, ES Cell Differentiation/IPS, GPCR, Transmembrane
Protein Pathways	Axon guidance, Chemokine signaling pathway, Cytokine-cytokine receptor interaction, Endocytosis, Leukocyte transendothelial migration

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