TCP1 alpha (TCP1) Rat Monoclonal Antibody [Clone ID: 91a]

Specifications

Product Data
Clone Name	91a
Applications	WB
Recommend Dilution	WB: 1:1000
Reactivity	Mouse, Rat, Rabbit, Hamster, Dog, Human, Pig, Saccharomyces cerevisiae, C. elegans, Monkey, Cow, Guinea Pig, Drosophila melanogaster
Host	Rat
Clonality	Monoclonal
Immunogen	Recombinant Mouse TCP1 alpha protein fragment (carboxy terminal region).
Formulation	PBS pH7.4, 50% glycerol, 0.1% sodium azide
Concentration	lot specific
Purification	Protein G Purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	t-complex 1
Database Link	NP_110379 Entrez Gene 21454 MouseEntrez Gene 24818 RatEntrez Gene 484064 DogEntrez Gene 702500 MonkeyEntrez Gene 6950 Human UniProt ID P17987
Background	T-complex polypeptide-1 (TCP1) is a ~60 kDa protein constitutively expressed in almost all eukaryotic cells, and is up-regulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin. The family of proteins termed chaperonins act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly, and share many characteristics with Heat Shock Protein 70 (HSP 70) including high abundance, induction by environmental stress, and ATPase activity. The chaperonin family includes the mitochondrial HSP60,Escherichia coli GroEL, the plastid Rubisco-subunit binding protein, and the archaebacterial protein TF55. The TCP1 sequence shows nearly 40% identity to TF55, but only minimal similarity to HSP60 and GroEL.
Synonyms	CCT-alpha; CCT1; CCTa; D6S230E; TCP-1-alpha
Note	Detects a 60kDa protein corresponding to the molecular weight of TCP1 alpha; also detects a molecular mass band of ~92kDa. Cross reactivity with human Hsp60 has been observed with this antibody in immunoblot analysis. Reacts weakly with Saccharomyces cerevisiae, consistent with the epitope sequence being AKLRS (instead of AKLRA). In C. elegans, it reacts with TCP1 alpha and another CCT subunit. In plants, it recognizes TCP1 of Pisum sativum, and the sequence of Arabidopsis thalania TCP1 over the region of the epitope AKLRA. It has also been shown that it reacts with a subunit of a specialized chaperonin which folds phytochrome.
Reference Data

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