Camk2a Mouse Monoclonal Antibody [Clone ID: 22B1]

Specifications

Product Data
Clone Name	22B1
Applications	IF, WB
Recommend Dilution	1u/ml was sufficient for detection of 0.2ug CAMKII by colorimetric immunoblot
Reactivity	Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptide
Formulation	PBS pH7.4, 50% glycerol
Concentration	lot specific
Purification	Protein G Purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	calcium/calmodulin-dependent protein kinase II alpha
Database Link	NP_037052 Entrez Gene 25400 Rat UniProt ID P11275
Background	CaMKII is an important member of calcium/calmodulin- activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling . CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site . The binding of Ca2+ auto inhibitory effect and activates the kinase . /calmodulin to its regulatory domain releases its This kinase activation results in autophosphorylation at threonine 286 . The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation . Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities .
Synonyms	CAMKA; CaMKIINalpha; KIAA0968
Note	Detects phosphorylated CaMKII from rat tissues. This antibody is specific for a and β subunits of CaMKII only when they are phosphorylated at Thr-286/287 (in β).
Reference Data

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