SIRT1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, WB
Recommend Dilution	WB 1:500 - 1:2000;IF 1:50 - 1:200
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	C term -peptide of human SIRT1
Formulation	Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Concentration	lot specific
Purification	Affinity purification
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	sirtuin 1
Database Link	NP_036370 Entrez Gene 93759 MouseEntrez Gene 23411 Human UniProt ID Q96EB6
Background	The Silent Information Regulator (SIR2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, also known as class III histone deacetylases. The first discovered and best characterized of these genes is Saccharomyces cerevisiae SIR2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response, and cell aging. SirT1, the mammalian ortholog of Sir2, is a nuclear protein implicated in the regulation of many cellular processes, including apoptosis, cellular senescence, endocrine signaling, glucose homeostasis, aging, and longevity. Targets of SirT1 include acetylated p53, p300, Ku70, forkhead (FoxO) transcription factors, PPAR?, and the PPAR? coactivator-1a (PGC-1a) protein. Deacetylation of p53 and FoxO transcription factors represses apoptosis and increases cell survival. Deacetylation of PPAR? and PGC-1a regulates the gluconeogenic/glycolytic pathways in the liver and fat mobilization in white adipocytes in response to fasting. SirT1 deacetylase activity is inhibited by nicotinamide and activated by resveratrol. In addition, SirT1 activity may be regulated by phosphorylation, since it is phosphorylated on Ser27 and Ser47 in vivo, however, the function of these phosphorylation sites has not yet been determined.
Synonyms	SIR2; SIR2alpha; SIR2L1
Reference Data
Protein Families	Druggable Genome, Stem cell - Pluripotency, Transcription Factors

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