Topoisomerase I (TOP1) Mouse Monoclonal Antibody [Clone ID: 1291CT875.142.166]

Specifications

Product Data
Clone Name	1291CT875.142.166
Applications	WB
Recommend Dilution	WB: 1:1000
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	This TOP1 antibody is generated from a mouse immunized with a recombinant protein from the N-terminal region of human TOP1.
Formulation	PBS with 0.09% (W/V) sodium azide
Concentration	lot specific
Purification	This antibody is purified through a protein G column, followed by dialysis against PBS.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	topoisomerase (DNA) I
Database Link	NP_003277 Entrez Gene 7150 Human UniProt ID P11387
Background	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.
Synonyms	TOPI
Reference Data
Protein Families	Druggable Genome

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