Calcium Sensing Receptor (CASR) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	FC, IF, WB
Recommend Dilution	WB: 1:200-1:2000; FC: 1:50-1:600
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide (C)DDYG RPGIE KFREE, corresponding to amino acid residues 216-229 of human CaSR.N-terminus, Extracellular.
Formulation	Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Reconstitution Method	Add 50 ul double distilled water (DDW) to the lyophilized powder.
Purification	Affinity purified on immobilized antigen.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	calcium sensing receptor
Database Link	NP_000379 Entrez Gene 12374 MouseEntrez Gene 24247 RatEntrez Gene 846 Human UniProt ID P41180
Background	The Calcium-Sensing Receptor (CaSR) is a member of subfamily C of the G-protein-coupled receptor (GPCR) superfamily. As its primary role, the CaSR is a key player in calcium homeostasis and is expressed in tissues involved in calcium metabolism, such as parathyroid cells and kidney. The expression of CaSR was also described in other cell types and tissues, such as neurons, keratinocytes, and pancreas. However, the role of CaSR in these cells remains to be established. Following the identification of functional vitamin D response elements in the CaSR gene, it was suggested that vitamin D might regulate the expression of CaSR. CaSR activation is followed by a rapid, transient increase in the cytosolic calcium concentration, resulting from mobilization of calcium from thapsigargin-sensitive intracellular stores, as well as an increased calcium influx through voltage-insensitive calcium channels located at the cell membrane. CaSR regulates cellular processes such as proliferation, apoptosis, and differentiation under both normal and pathologic conditions. Recent studies demonstrated the expression in human colon epithelium of CaSR, which regulates proliferation and differentiation. In colon carcinomas,lower levels of expression were found in compared to normal colon tissue.
Synonyms	CAR; EIG8; FHH; FIH; GPRC2A; HHC; HHC1; HYPOC1; NSHPT; PCAR1
Reference Data
Protein Families	Druggable Genome, GPCR, Transmembrane

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