C5R1 (C5AR1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	FC, WB
Recommend Dilution	WB: 1:200-1:2000; IHC: 1:100-1:3,000; FC: 1:50-1:600
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide (C)DKDTLDLNTPVDK, corresponding to amino acid residues 16-28 of human C5aR (Accession P21730 ). Extracellular, N-terminus.
Formulation	Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Reconstitution Method	Add 50 ul double distilled water (DDW) to the lyophilized powder.
Purification	Affinity purified on immobilized antigen.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	complement component 5a receptor 1
Database Link	NP_001727 Entrez Gene 728 Human UniProt ID P21730
Background	The innate immune system identifies pathogens by recognizing known elements. This response ultimately leads to the production of proinflammatory substances and the activation of phagocytic neutrophils and macrophages. Activation of the complement system is an important initial even in the response against pathogens. This system includes some thirty different proteins that act together to form a complex. C5a is one of the most potent inflammatory peptides generated by complement activation. This peptide has many functions such as acting as a chemoattractant for neutrophils. C5a acts as a vasodilator and activates the coagulation pathway just to name a few. C5a exerts its numerous actions by binding to C5aR and C5L2. C5aR belongs to the superfamily of G-protein coupled receptors (GPCRs). Like all members, C5aR has seven transmembrane domains, an extracellular N-terminus and an intracellular C-terminal tail. C5aR can form homodimers or heteromer with CCR5, chemokine receptor, for example. Activation of C5aR by C5a binding causes Ca2+ mobilization. C5aR expression is detected in neutrophils, eisinophils, basophils and monocytes. The receptor is also expressed in lung and liver. Blocking C5aR by peptides or organic small molecules is in the pipeline for treating chronic inflammatory disorders/diseases like arthritis, inflammatory bowel disease and lung/liver injuries.
Synonyms	C5A; C5AR; C5R1; CD88
Reference Data
Protein Families	Druggable Genome, GPCR, Transmembrane
Protein Pathways	Complement and coagulation cascades, Neuroactive ligand-receptor interaction

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