CLIC4 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, WB
Recommend Dilution	WB: 1:200-1:2000; FC: 1:50-1:600
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide (C)NGLKEEDKEPLIE, corresponding to amino acid residues 8 - 20 of human CLIC4. Intracellular, N-terminus.
Formulation	Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Reconstitution Method	Add 50 ul double distilled water (DDW) to the lyophilized powder.
Purification	Affinity purified on immobilized antigen.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	chloride intracellular channel 4
Database Link	NP_039234 Entrez Gene 29876 MouseEntrez Gene 83718 RatEntrez Gene 25932 Human UniProt ID Q9Y696
Background	Chloride intracellular channel (CLIC) is a family of proteins encoded by six different genes (CLIC1-6). The localization of the various CLIC channels remains elusive and controversial since they can be either found in a soluble form in the cytoplasm or as integral membrane proteins (in intracellular compartments such as the nuclear membrane or the endoplasmic reticulum for example, or associate to the plasma membrane) via their single transmembrane domain. When membrane associated, CLIC channels indeed function as ion channels (enabling the passage of Cl ions). Non-channel functions are also attributed to CLICs and include cell cycle regulation, cell differentiation, and apoptosis. These different roles depend whether CLICs are membrane bound or cytosolic. For instance, CLIC4 is involved in keratinocyte differentiation in a TGFb-dependent manner, in such a way that TGFb stimulation induces the expression and nuclear localization of CLIC4 and its subsequent interaction with transcription factors. CLIC4 is also required for growth arrest and apoptosis under stress. CLIC4 distribution is rather ubiquitous but shows higher expression level in skin, kidney, brain and testes.
Synonyms	CLIC4L; H1; huH1; MTCLIC; p64H1
Reference Data
Protein Families	Druggable Genome, Ion Channels: Other

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