CNTF Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	WB: 1:200-1:2000; IHC: 1:100-1:3000
Reactivity	Human, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide (C)ELSQWTVRSIHCDLR, corresponding to amino acid residues 164-177 of human CNTF. C-terminus.
Formulation	Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS) pH 7.4, 1% BSA, 0.05% NaN3.
Reconstitution Method	Add 50 ul double distilled water (DDW) to the lyophilized powder.
Purification	Affinity purified on immobilized antigen.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	ciliary neurotrophic factor
Database Link	NP_000605 Entrez Gene 25707 RatEntrez Gene 1270 Human UniProt ID P26441
Background	CNTF is a polypeptide trophic factor, member of the alpha-helical cytokine superfamily. It was initially purified from the chick eye on the basis of its ability to promote survival of E8 chick ciliary ganglion neurons in culture. CNTF is synthesized by glia both in the CNS and PNS and it has been demonstrated that CNTF is ubiquitously distributed in neurons and glia throughout the rodent brain. CNTF effects are mediated by a tripartite receptor complex consisting of two signal-transducing subunits (leukemia inhibitory factor receptor, gp130) and a CNTF-specific ligand-binding-subunit (CNTFR). CNTF can support the survival of many different cell populations within the PNS and the CNS. in vitro, CNTF promotes proliferation and neuronal specifications in hippocampal neurons. in vivo, it supports the viability of non-primate motor neurons, CNTF induces sprouting of cholinergic motor neurons and delays neural degeneration in genetic models of motor neuron disease. In addition, it is involved in the development stage of astrocytes and oligodendocytes.
Synonyms	HCNTF
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway

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