Cacnb1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	WB: 1:200-1:2000; IHC: 1:100-1:3000
Reactivity	Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide (C)DRATGEHASVHEYPGE, corresponding to amino acid residues 456-471 of rat CaVÃ?1. Intracellular, adjacent to the C-terminus.
Formulation	Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Reconstitution Method	Add 50 ul double distilled water (DDW) to the lyophilized powder.
Purification	Affinity purified on immobilized antigen.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	calcium voltage-gated channel auxiliary subunit beta 1
Database Link	NP_059042 Entrez Gene 12295 MouseEntrez Gene 50688 Rat UniProt ID P54283
Background	Voltage-gated Ca2+ (CaV) channels are ubiquitously expressed and function as Ca2+ conducting pores in the plasma membrane1. Based on their electrophysiological and pharmacological properties, CaV channels have traditionally been classified into L, T, N, P, Q, and R types. L-type Ca2+ channels are heteromultimers composed of four independently encoded proteins, the pore-forming a1 subunit, which triggers Ca2+ flow across the membrane, and the subunits a2d, ?, and Ã?.CaVÃ? subunits play critical roles in membrane trafficking of the channel complex and regulation of voltage-dependent gating. The CaVÃ? subunit binds to the endoplasmic reticulum (ER) retention signal in the I-II loop of the a1-subunit, which allows channels to traffic to the surface membrane4. Furthermore, CaVÃ? subunits not only allow for membrane trafficking of the channel complex, they also can play a role in determining the subcellular localization of channels on the surface membrane. There are four distinct CaVÃ? subunits CaVÃ?1, CaVÃ?2, CaVÃ?3 and CaVÃ?4.Three splice variants exist for the Ã?1 subunit: Ã?1a, Ã?1b and Ã?1c. Ã?1a is known to be expressed in skeletal muscle and brain, but not in smooth muscle or heart. Ã?1a appears to be important for the functional expression of the a1 subunit in skeletal muscle. Ã?1b was identified by cloning in rat brain, heart and hippocampus, and differs from Ã?1a by having a deletion of ~50 amino acids at residue 209, and having a 120-residue C-terminal elongation. Ã?1c was cloned from human heart and hippocampus and has the same deletion as Ã?1b, but lacks the C-terminal extension.
Synonyms	CAB1; CACNLB1; CCHLB1; MGC41896
Reference Data

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