Catsper1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	WB: 1:200-1:2000
Reactivity	Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide (C)RALFQDSDPKRFQN, corresponding to amino acid residues 508-521 of mouse CatSper1. 3rd extracellular loop.
Formulation	Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Reconstitution Method	Add 50 ul double distilled water (DDW) to the lyophilized powder.
Purification	Affinity purified on immobilized antigen.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	cation channel, sperm associated 1
Database Link	NP_647462 Entrez Gene 689349 RatEntrez Gene 225865 Mouse UniProt ID Q91ZR5
Background	Capacitation is the final stage of spermatozoon maturation which eventually enables it to bind the zona pellucida (ZP) - a thick layer of glycoproteins surrounding the oocyte. It occurs in the fallopian tubes of the female reproductive tract and is preceded by spermatogenesis (testes) and epididymal maturation (epididymis). Capacitation is accompanied by drastic morphological and functional alterations, such as membrane hyperpolarization, changes in membrane lipid composition, intracellular alkalinization, increased level of protein tyrosine phosphorylation by cAMP-dependent PKA, and elevated intracellular concentrations of Ca2+ ([Ca2+]i) and pHi, which render the sperm chemotaxis-responsive and hyperactive. Increased flagellar bend, triggered by Ca2+ signaling, produces asymmetrical, whiplash-like motility pattern that provides the sperm with significantly stronger swimming thrust. Hyperactivity, together with the eruption of hydrolytic enzymes from the sperm's acrosome vesicle facilitate the disruption of the ZP matrix and make way for the sperm to enter the egg cytoplasm. CatSper (cation channel of spermatozoa) is a sperm-specific Ca2+ channel located explicitly in the spermatozoon flagellum2. It mediates flagellar hyperactivity, and is chemotactically responsive to progesterone. CatSper is composed of 4 pore-forming subunits (CatSper1-4) with 6 transmembrane (TM) domains each, and of 3 auxiliary subunits: CatSperÃ? (2 TM domains), CatSper? and CatSperd (1 TM domain each). While all four CatSper proteins are necessary for the channel's function (and hence male fertility)9, each is synthesized independently. Trafficking to the flagellum requires the cooperation of all subunits. CatSper1, the first identified subunit of the channel, is highly homologous to CaV channels; a voltage sensor domain (S1-S4) containing enough positively charged residues to confer weak voltage sensitivity to the channel. Furthermore, its histidine-rich, intracellular amino terminus likely serves as the pH sensor necessary to induce hyperactivation (by permitting an influx of Ca2+). Indeed, intracellular alkaline pH (7.9-8.5) was shown to efficiently hyper-activate CatSper channels.
Synonyms	CATSPER; hCatSper; MGC33335; MGC33368
Reference Data

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