Drd4 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	WB: 1:200-1:2000
Reactivity	Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide (C)RRWEAARHTKLHSR, corresponding to amino acid residues 215-228Â of rat D4 Dopamine receptor. 3rd intracellular loop.
Formulation	Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
Reconstitution Method	Add 50 ul double distilled water (DDW) to the lyophilized powder.
Purification	Affinity purified on immobilized antigen.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	dopamine receptor D4
Database Link	NP_037076 Entrez Gene 13491 MouseEntrez Gene 25432 Rat
Background	The Dopamine neurotransmitter belongs to catecholamines and can therefore be further converted into adrenaline and noreadrenaline. Dopamine has various physiological roles, including learning and memory, motor output and endocrine regulation. It does so by binding and activating Dopamine receptors which belong to the G-protein coupled receptor superfamily (GPCR). The D4 Dopamine Receptor belongs to the D2-like family as do D2 and D3 Dopamine Receptors and like all GPCRs has seven transmembrane spanning membrane regions. Structure wise members of the family share high homology in the transmembrane domains and lower homology in the extracellular N-Terminal and the intracellular C-terminal domains. Notably, the coding region of the 3rd intracellular loop of D4 Receptor is known to undergo extensive polymorphism. Like many GPCRs, each dopamine receptor subtype can react with more than one G-protein giving rise to different signaling possibilities. Whereas D2-like dopamine receptors are generally considered to couple to Gi, and therefore inhibit adenylyl cyclase, the signaling through D4 is complicated due to the polymorphisms in the 3rd intracellular loop. It seems that this region is important to G-coupling as different polyphormisms in the region influence the ability of D4 to couple to adenylyl cyclase and G-proteins. D4 Dopamine Receptors also influence Ca2+ levels. They could also interact with G-protein couple inwardly rectifying K+ channel to ultimately cause a decrease in the firing rate of neurons. The distribution of D4 Dopamine Receptor mostly includes the brain and is mainly found post-synaptically in dendritic shafts and spines of mammalian striatum.
Synonyms	D4DR
Reference Data

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