Ghsr Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, WB
Recommend Dilution	WB: 1:200-1:2000; FC: 1:50-1:600
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide CRATEFAVRSG, corresponding to amino acid residues 197-207 of rat Ghrelin Receptor. 2nd extracellular loop.
Formulation	Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Reconstitution Method	Add 50 ul double distilled water (DDW) to the lyophilized powder.
Purification	Affinity purified on immobilized antigen.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	growth hormone secretagogue receptor
Database Link	NP_114464 Entrez Gene 2693 HumanEntrez Gene 208188 MouseEntrez Gene 84022 Rat UniProt ID O08725
Background	Ghrelin is a 28 amino acid peptide, acylated on a serine residue which is essential for its biological activity. In rodents, an additional form of the peptide could be purified. The peptide is released from the stomach and exerts its effect to ghrelin receptor (GHS-R). GHS-R is a member of the G-protein coupled receptor (GPCR) superfamily. Like all members, the receptor has seven transmembrane spanning domains and upon activation it stimulates phospholipase C, thereby increasing IP3 and intracellular Ca2+ levels. GHS-R is expressed in the hypothalamus, pituitary and hippocampus. Some of the actions of ghrelin include the stimulation of growth hormone release, food intake, fat accumulation, regulation of memory and learning, activation of reward-related pathways and neuroprotection to name a few. GHS-R 1b is a truncated form of the full length receptor which is also expressed. Although its exact functions are unknown, it is believed that it may be involved in downregulating signaling from the full length receptor. In addition, it is overexpressed in lung cancer where it dimerizes with neurotensin receptor.
Synonyms	GHRP; GHS-R; OTTHUMP00000211093
Note	This antibody was tested in live cell imaging. Please see IF/ICC data for detail.
Reference Data

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