Adgrl1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	WB: 1:200-1:2000; IHC: 1:100-1:3000
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide CEPREVRRVQWPATQ(G), corresponding to amino acid residues 480-494 of rat Latrophilin-1. Extracellular, N-terminus.
Formulation	Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Reconstitution Method	Add 50 ul double distilled water (DDW) to the lyophilized powder.
Purification	Affinity purified on immobilized antigen.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	adhesion G protein-coupled receptor L1
Database Link	NP_075251 Entrez Gene 22859 HumanEntrez Gene 330814 MouseEntrez Gene 65096 Rat UniProt ID O88917
Background	Latrophilins (Latrophilin1-3) are members of the adhesion G-protein coupled receptor subfamily. Like all GPCRs, Latrophilins have seven transmembrane domains and are distinguished by a large extracellular N-terminal tail and a large intracellular C-terminal tail1. The N-terminus has several cell adhesion domains and undergoes proteolysis after synthesis, while the C-terminal has various consensus post-translational sites like phosphorylation and palmitoylation. In addition, Latrophilins undergo alternative splicing.Latrophilin-1 was discovered by its ability to bind a-Latrotoxin (a-LTX), a toxin isolated from the black widow spider venom. a-LTX induces exocytosis by creating a Ca2+ influx in the presynaptic membrane. a-LTX can also stimulate small vesicle exocytosis in a Ca2+ independent manner. Three receptors have been found to bind a-LTX. Of the three, Latrophilins are responsible for the Ca2+-independent effects of a-LTX2. The binding of a-LTX to Latrophilin-1 increases exocytosis of neurotransmitters.In an attempt to find the natural ligand of Latrophilin-1, Lasso, a splice variant of Teneneurin-2 was discovered to be an endogenous binding partner of the adhesion-GPCR7. Teneneurins are large glycoproteins with a single transmembrane domain. Like Latrophilin-1, Teneneurins are mostly expressed in the brain where they modulate neurite outgrowth, axon guidance and synaptogenesis.Regarding the localization of Latrophilins, Latrophilin-1 is expressed predominantly in the brain, Latrophilin-2 is highly expressed in the liver and lung, while Latrophilin-3 is almost exclusively detected in the brain.
Note	This antibody was tested in live cell imaging. Please see IF/ICC data for detail.
Reference Data

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