Nrxn1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	WB: 1:200-1:2000; IHC: 1:100-1:3,000; FC: 1:50-1:600
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide (C)KPRHQKDAKHPQMIK, corresponding to amino acid residues 546-560 of rat Neurexin 1a . Extracellular, N-terminus.
Formulation	Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Reconstitution Method	Add 50 ul double distilled water (DDW) to the lyophilized powder.
Purification	Affinity purified on immobilized antigen.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	neurexin 1
Database Link	NP_068535 Entrez Gene 9378 HumanEntrez Gene 18189 MouseEntrez Gene 60391 Rat UniProt ID Q63372
Background	Neurexins (NRXNs) are a family of transmembrane, synaptic adhesion molecules. NRXNS were identified as receptors for a-latrotoxin, a presynaptic toxin that triggers massive neurotransmitter release1. Neurexins are largely presynaptic proteins that form a trans-synaptic cell-adhesion complex with postsynaptic neuroligins. They are encoded by three genes (NRXN1, NRXN2 and NRXN3), each using an upstream promoter to produce the longer a-isoform (a-NRXNs) and a downstream promoter to generate a shorter �-isoform (�- NRXNs) .The a-isoforms and �-isoforms of each neurexin are single-pass transmembrane proteins maintaining identical transmembrane and intracellular domains but having distinct extracellular domains. NRXNs in neurons localize to the presynaptic membrane and bind trans-synaptically to postsynaptic adhesion molecules and receptors. Neurexins are expressed in all neurons, and are subject to extensive alternative splicing, generating >1,000 splice variants, some of which exhibit highly regulated developmental and spatial expression patterns. The extracellular domain of neurexin 1a (NRXN1a) is composed of three neurexin repeats (I, II, and III), which each contains the modules LNS-EGF-LNS. NRXN1a binds endogenous ligands including neuroligins, LRRTM family members, neurexophilin, a-dystroglycan, and GABA(A) receptors. NRXN1a gene mutations have been identified in neuropsychiatric diseases including Schizophrenia (SCZ) and Autism Spectrum Disorder (ASD).
Synonyms	DKFZp313P2036; FLJ35941; KIAA0578; OTTHUMP00000200525
Note	This antibody was tested in live cell imaging. Please see IF/ICC data for detail.
Reference Data

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