Slc4a4 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	WB: 1:200-1:2000; IHC: 1:100-1:3000
Reactivity	Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide (C)ENMGAEFLESMEEGR, corresponding to amino acid residues 22-36 of rat Concentrative Nucleoside Transporter 1 . Intracellular, N-terminus.
Formulation	Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Reconstitution Method	Add 50 ul double distilled water (DDW) to the lyophilized powder.
Purification	Affinity purified on immobilized antigen.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	solute carrier family 4 member 4
Database Link	NP_445876 Entrez Gene 54403 MouseEntrez Gene 84484 Rat UniProt ID Q9JI66
Background	SLC4 membrane transporter proteins play important roles in kidney acidâ??base regulation through their transport of bicarbonate (or carbonate), Na?+, Cl-, and (possibly NH4+)1. These transporters differ in their substrate (Na+?, Cl-) dependence, charge transport stoichiometry, cell-type and developmental expression, functional regulation, and proteinâ??protein interactions. In mammals, SLC4 proteins are encoded by 10 different genes that share protein sequence homology and are grouped according to their functional properties.In humans, the electrogenic sodium bicarbonate cotransporter NBCe-1 is encoded by the SLC4A4 gene. NBCe1-A has a large N-terminal cytoplasmic region, a lipid embedded transmembrane region, and a C-terminal cytoplasmic tail. The N-terminal cytoplasmic region is tightly folded and is predicted to form a domain structure, unlike the freely aqueous accessible C-terminal cytoplasmic tail. NBCe1-A is composed of either 10 or 14 transmembrane domains. Based on the 10 transmembrane topology, a large extracellular loop (EL) is present between TM3 and TM4 and contains two glycosylated sites. The oligomeric state of the cotransporter is dimeric and each monomeric subunit has independent transport activity.Mutations in NBCe1 cause autosomal recessive proximal renal tubular acidosis (pRTA). Patients with NBCe1 mutations have severe pRTA, growth and mental retardation, basal ganglia calcification, cataracts, corneal opacities (band keratopathy), glaucoma, elevated serum amylase and lipase, and defects in the enamel consistent with amelogenesis imperfect. Mice with loss of NBCe1 have a more severe phenotype with marked volume deletion and decreased survival.
Synonyms	DKFZp781H1314; hhNMC; HNBC1; KNBC; kNBC1; NBC; NBC1; NBC2; NBCE1; pNBC; SLC4A5
Note	This antibody was tested in live cell imaging. Please see IF/ICC data for detail.
Reference Data

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