Slc9a1 Rabbit Polyclonal Antibody
CAT#: TA328914
Rabbit polyclonal Anti-Na+/H+ Exchanger 1 (NHE-1) (extracellular)
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CNY 11,000.00
货期*
4周
规格
Cited in 5 publications. |
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Specifications
Product Data | |
Applications | FC, IF, WB |
Recommend Dilution | WB: 1:200-1:2000; FC: 1:50-1:600 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | Peptide (C)RERSIGDVTTAPSE, corresponding to amino acid residues 54-67 of rat NHE-1 . 1st extracellular loop. |
Formulation | Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3. |
Reconstitution Method | Add 50 ul double distilled water (DDW) to the lyophilized powder. |
Purification | Affinity purified on immobilized antigen. |
Conjugation | Unconjugated |
Storage Condition | Store at -20°C as received. |
Gene Name | solute carrier family 9 member A1 |
Database Link | |
Background | In order to function in optimal conditions, cells must maintain a close to neutral intracellular pH. They have adopted various mechanisms in order to do so, one of which is via Na+/H+Â exchangers (NHEs). Genes belonging to this group are expressed along a very broad range of organisms and are essential for protecting cells against intracellular acidification.To date, nine genes have been identified in mammals; NHE1-9. These membrane proteins have 10-12 transmembrane domains depending on whether a splice variant is expressed and an intracellular N-terminal. The C-terminal domain can be either intracellular or extracellular, also depending whether a splice variant of the protein is involved. The C-terminal part of the protein also undergoes posttranslational modification such as phosphorylation.Both NHE-1 and NHE-2 have an extracellular loop which is glycosylated.Under physiological conditions, the Na+/H+ exchanger mediates the exchange of one extracellular Na+ ion for one intracellular proton, thereby keeping the overall charge neutral1. The extracellular binding site of Na+ is not selective as it can also bind Li+ and H+ .K+ ions inhibit NHE-1 but have no effect on NHE-2.The activation of NHE-1 and NHE-2 is sensitive to intracellular acidic pH. Under physiological conditions, both exchangers are not active and upon a drop of intracellular pH, they are rapidly activated.NHE-1 expression is ubiquitous and may serve as a housekeeping gene. |
Synonyms | APNH; APNH1; FLJ42224; NHE-1; NHE1 |
Reference Data |
Citations (5)
The use of this Antibodies has been cited in the following citations: |
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Characterization of intracellular buffering power in human induced pluripotent stem cells and the loss of pluripotency is delayed by acidic stimulation and increase of NHE1 activity
,Lee, SP;Chao, SC;Chou, MF;Huang, SF;Dai, NT;Wu, GJ;Tsai, CS;Loh, SH;Tsai, YT;,
J. Cell. Physiol.
,PubMed ID 32841374
[SLC9A1]
|
Effects of Andrographolide on Intracellular pH Regulation, Cellular Migration, and Apoptosis in Human Cervical Cancer Cells †
,Loh, SH;Tsai, YT;Huang, SF;Yu, TC;Kuo, PC;Chao, SC;Chou, MF;Tsai, CS;Lee, SP;,
Cancers (Basel) 2020
,PubMed ID 32046125
[SLC9A1]
|
Acidic Stress Triggers Sodium-Coupled Bicarbonate Transport and Promotes Survival in A375 Human Melanoma Cells
,Yang, OCY;Loh, SH;,
Sci Rep
,PubMed ID 31048755
[SLC9A1]
|
Functional and molecular characterization of transmembrane intracellular pH regulators in human dental pulp stem cells.
,null,
Archives of oral biology
,PubMed ID 29524788
[Slc9a1]
|
Expressional and Functional Characterization of Intracellular pH Regulators and Effects of Ethanol in Human Oral Epidermoid Carcinoma Cells
,Lee, SP;Chao, SC;Huang, SF;Chen, YL;Tsai, YT;Loh, SH;,
Cell. Physiol. Biochem.
,PubMed ID 29975935
[SLC9A1]
|
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