Kcnk18 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	WB: 1:200-1:2000; IHC: 1:100-1:3000
Reactivity	Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide (C)EAEENPELKKFLDD, corresponding to amino acid residues 62-75 of mouse K2P18.1. 1st extracellular loop.
Formulation	Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
Reconstitution Method	Add 50 ul double distilled water (DDW) to the lyophilized powder.
Purification	Affinity purified on immobilized peptide.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	potassium channel, subfamily K, member 18
Database Link	NP_997144 Entrez Gene 445371 RatEntrez Gene 332396 Mouse UniProt ID Q6VV64
Background	K2P18.1 (also named TWIK-related spinal cord K+ channel, TRESK or KCNK18) is a member of the 2-pore (2P) domain K+ channels family that in mammals includes 15 members. These channels show little time or voltage dependence and are considered to be â??leakâ?? or â??backgroundâ?? K+ channels, thereby generating background currents which help set the membrane resting potential and control cell excitation. The K2P channels have a signature topology that includes four transmembrane domains and two pore domains with intracellular N- and C termini. It has been proposed that the functional channel unit is a dimer. Different K2P family members are regulated by diverse physical and chemical stimuli including temperature, pH, mechanical stretch, inhalation anesthetics, signaling pathways (PKC and PKA), arachidonic acid, etc. K2P18.1 is the only K2P channel so far, whose current is activated following Gaq-receptor coupled activation. The enhancement of K2P18.1 current involves activation of calcineurin (calciumâ??calmodulin-dependent phosphatase 2B) following the rise in intracellular calcium that occurs subsequent to Gaq activation. In addition, K2P18.1 is potently activated by clinical concentrations of volatile anesthetics. K2P18.1 expression in humans is largely restricted to the spinal cord although in rodents it has a broader expression pattern that includes brain, testis and spleen. K2P18.1 represents the most important background K+ channel in dorsal root ganglion neurons and hence it has been postulated that it has an important role in acute and chronic pain as well as general anesthesia.
Synonyms	K2p18.1; TRESK; TRESK-2; TRESK2; TRIK
Reference Data

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