Scnn1g Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	WB: 1:200-1:2000
Reactivity	Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide (C)YGVKESRKRREAGS, corresponding to amino acid residues 129-142 of rat ENaC?. Extracellular.
Formulation	Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
Reconstitution Method	Add 50 ul double distilled water (DDW) to the lyophilized powder.
Purification	Affinity purified on immobilized antigen.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	sodium channel epithelial 1 gamma subunit
Database Link	NP_058742 Entrez Gene 24768 Rat UniProt ID P37091
Background	The amiloride-sensitive epithelial Na+ channel (ENaCs) family includes 4 members: ENaCα, β, γ and δ. The ENaC channel is located in the luminal (apical) plasma membrane of several epithelial tissues such as kidney, lung, salivary glands and skin. The functional channel is believed to be a multimer including a α (or δ) and a β and γ subunits with a likely stoichiometry of α2βγ. ENaC channel enable entry of Na+ into the cell along its electrochemical gradient and thus has a central role in the maintenance of renal Na+ balance (and hence blood pressure) and liquid balance in the lung. Indeed, genetic mutations in the ENaC subunits causes Liddle’s syndrome (a form of hypertension) or pseudohypoaldosteronism type 1 (PHA) that is characterized by hypotension. The ENaC channel is voltage-independent and is constitutively active in epithelia although it is modulated by several different mechanisms. One of the main mechanisms is the controlled internalization of the channel that is dependent on the β or γ subunits. Indeed, mutations in the C-termini of these subunits reduce endocytosis of the channel leading to the accumulation of ENaC in the cell membrane and causing a phenotype that is consistent with that of Liddle’s syndrome.
Synonyms	BESC3; ENaCg; ENaCgamma; Gamma-ENaC; Gamma-NaCH; PHA1; SCNEG
Reference Data

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