Cullin 3 (CUL3) Goat Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	WB: 1-3ug/ml, ELISA: 1:2,000
Reactivity	Human, Mouse, Rat
Host	Goat
Clonality	Polyclonal
Immunogen	Internal region (AQVTGSNTRKHILQ)
Formulation	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration	lot specific
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	cullin 3
Database Link	NP_003581 Entrez Gene 26554 MouseEntrez Gene 301555 RatEntrez Gene 8452 Human UniProt ID Q13618
Background	Cullin proteins assemble a large number of RING E3 ubiquitin ligases and regulate various physiological processes (1). Cullin 3-based E3 ligase (CUL3) forms a catalytically inactive BTB-CUL3-Rbx1 (BCR) ubiquitin ligase, which becomes functional upon covalent attachment of the ubiquitin Nedd8 (neural precursor cell-expressed, developmentally downregulated 8) near the C terminus of CUL3 (2). CUL3 plays an essential role in both axonal arborization and proper elaboration of dendrites and may require neddylation for its proper function (3). The CUL3 pathway, which selects cyclin E for ubiquitination on the basis of its assembly into CDK complexes, may be one way to control cyclin abundance (4). In a complex with the substrate-specific adaptors KLHL9 and KLHL13, CUL3 is required for correct chromosome alignment in metaphase, proper midzone and midbody formation, and completion of cytokinesis (5).
Synonyms	CUL-3; PHA2E
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Ubiquitin mediated proteolysis

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